說明
This report summarizes results from water samples collected from 105 ponds in Southern Norway in 2019 to determine amphibian biodiversity in the ponds.
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GBIF 註冊
此資源尚未向GBIF註冊
關鍵字
eDNA; water sample; Amphibian species; Occurrence
聯絡資訊
- 元數據提供者 ●
- 連絡人
- Molecular biologist
- c/o Göteborgs Universitet, , Carl Skottsbergs gata 22B
- 0700264843
- 元數據提供者 ●
- 連絡人
- c/o Göteborgs Universitet, , Carl Skottsbergs gata 22B
- 0700264843
地理涵蓋範圍
A selected number of systems was sampled during different seasons to provide the necessary basis for an optimal sampling design for a national monitoring program of BD and amphibian diversity in southern Norway.
| 界定座標範圍 | 緯度南界 經度西界 [36.598, -4.219], 緯度北界 經度東界 [77.466, 35.156] |
|---|
分類群涵蓋範圍
Animalia;Chordata;Amphibia;Anura;Bufonidae;Bufo;Bufo_bufo Animalia;Chordata;Amphibia;Caudata;Salamandridae;Lissotriton;Lissotriton_vulgaris Animalia;Chordata;Amphibia;Anura;Ranidae;Rana;Rana_arvalis Animalia;Chordata;Amphibia;Anura;Ranidae;Rana;Rana_temporaria Animalia;Chordata;Amphibia;Anura;Bufonidae;Epidalea;Epidalea_calamita Animalia;Chordata;Amphibia;Anura;Ranidae;Pelophylax;Pelophylax_esculentus Animalia;Chordata;Amphibia;Caudata;Salamandridae;Triturus;Triturus_cristatus
| Species | Rana_temporaria, Lissotriton_vulgaris, Bufo_bufo, Rana_arvalis, Epidalea_calamita, Pelophylax_esculentus, Triturus_cristatus |
|---|
時間涵蓋範圍
| 起始日期 / 結束日期 | 2019-05-26 / 2019-09-08 |
|---|
計畫資料
The main objectives of this project were to collect samples from a high number of waterbodies to obtain a detailed picture of the presence of BD and amphibians in southern Norway. Furthermore, a selected number of systems was sampled during different seasons to provide the necessary basis for an optimal sampling design for a national monitoring program of BD and amphibian diversity.
| 計畫名稱 | Monitoring and mapping of amphibian species using environmental DNA |
|---|---|
| 辨識碼 | eDNA |
| 經費來源 | The project was funded by the Norwegian Environmental Agency "Miljødirektoratet" |
| 研究區域描述 | Samples were obtained in Southern Norway, along the Swedish border, around Oslo, and in the Agder region. Based on the results from the 2019 study (Ahmed et al., 2019), the end of May and beginning of June were chosen for sampling. In total, 91 bodies of water, including forest-, agricultural- and urban ponds, were sampled, and 16 swabs from various amphibian specimens were obtained in 2020. |
| 研究設計描述 | 300-500 milliliters of water were filtered through sterivex filters. |
參與計畫的人員:
取樣方法
Water samples were collected using 50 ml syringe and Stervix filter units. Approximately 100-600 ml of water was injected in Stervix filter.
| 研究範圍 | Norway |
|---|---|
| 品質控管 | Negative controls were prepared and also positive control. PCRs were run in triplicates. |
方法步驟描述:
- dx.doi.org/10.17504/protocols.io.973h9qn